Biologics

Authors: Rialland P, Bichot S, Lussier B, Moreau M, Beaudry F, Del Castillo JR, Gauvin D, Troncy E.
Journal: Can J Vet Res

This study aimed to establish the effect of a diet enriched with green-lipped mussel (GLM) on pain and functional outcomes in osteoarthritic dogs. Twenty-three client-owned dogs with osteoarthritis (OA) were fed a balanced control diet for 30 d and then a GLM-enriched balanced diet for the next 60 d. We assessed peak vertical force (PVF), which is considered to be the gold standard method, at Day (D)0 (start), D30 (end of control diet), and D90 (end of GLM-enriched diet). The owners completed a client-specific outcome measure (CSOM), which is a pain questionnaire, once a week.

Authors: Dycus DL, Au AY, Grzanna MW, Wardlaw JL, Frondoza CG.
Journal: AJVR

Objective-To determine whether oxidative stress could be induced in canine chondrocytes in vitro. Sample-Chondrocytes obtained from healthy adult mixed-breed dogs. Procedures-Harvested chondrocytes were maintained at 37°C with 5% CO2 for 24 hours. To assess induction of oxidative stress, 2 stimuli were used: hydrogen peroxide and a combination of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). To determine the effect of hydrogen peroxide, a set of chondrocyte-seeded plates was incubated with control medium alone or hydrogen peroxide (100, 200, or 300μM) for 24 hours.

Authors: Case JB, Palmer R, Valdes-Martinez A, Egger EL, Haussler KK.
Journal: Vet Surg

OBJECTIVE:
To report clinical findings and outcome in a dog with gastrocnemius tendon strain treated with autologous mesenchymal stem cells and a custom orthosis.
STUDY DESIGN:
Clinical report.
ANIMAL:
A 4-year-old spayed female Border Collie.
METHODS:
Bone-marrow derived, autologous mesenchymal stem cells were transplanted into the tendon core lesion. A custom, progressive, dynamic orthosis was fit to the tarsus. Serial orthopedic examinations and ultrasonography as well as long-term force-plate gait analysis were utilized for follow up.
RESULTS:

Authors: Krontveit RI, Sævik BK.
Journal: Vet J

No abstract available

Authors: Akagi H, Ochi H, Kannno N, Iwata M, Ichinohe T, Harada Y, Nezu Y, Yogo T, Tagawa M, Hara Y.
Journal: VCOT

Objectives: To evaluate the efficacy of cortical allograft and fibroblast growth factor 2 (FGF-2)-impregnated autogenous cancellous bone in nonunion fracture repair in dogs. Methods: From January 2000 to August 2010, seven dogs underwent cortical allograft and FGF-2-impregnated autogenous cancellous bone implantation for treatment of a femoral nonunion following fracture. Radiographic images were used to assess healing. Results: The average length of the implanted cortical allograft was 29.1 ± 4.4 mm.

Authors: Corbin EE, Cavanaugh RP, Fick JL, McAbee KP, Powers B.
Journal: VCOT

A dog was presented with the complaint of an acute onset left pelvic limb lameness three years after a right tibial plateau levelling osteotomy had been performed. Radiographs taken at the time of presentation showed signs that were consistent with a diagnosis of an implant associated sarcoma. At revision surgery, a retained surgical sponge was identified, leading to a diagnosis of a gossypiboma. This is the first reported case of a gossypiboma as a complication of a tibial plateau levelling osteotomy surgery.

Authors: Lappalainen AK, Mölsä S, Liman A, Snellman M, Laitinen-Vapaavuori O.
Journal: JSAP

OBJECTIVE:
To determine whether the current Finnish screening method using a single flexed mediolateral view as scored by osteophyte is sufficient to diagnose mild elbow dysplasia in Labrador retrievers and to determine if an additional craniocaudal oblique projection would result in improvement in the screening protocol.
MATERIALS AND METHODS:
Thirteen dogs with one mildly affected elbow joint and one elbow joint without radiological evidence of osteophytes were studied. Radiographic and computed tomography studies were performed and the results compared with each other.
RESULTS:

Category: Biologics - Hip - Imaging
Authors: Zhang N, Dietrich MA, Lopez MJ.
Journal: Vet Surg

OBJECTIVE:
To identify the optimum intra-articular multipotent stromal cell (MSC) tissue source in the canine stifle.
STUDY DESIGN:
Experimental.
SAMPLE POPULATION:
Infrapatellar adipose tissue, synovium lining the joint capsule, and synovium surrounding the cranial cruciate ligament (CrCL) from normal stifles of 6 dogs.
METHODS:

Authors: Pinel CB, Pluhar GE.
Journal: Can Vet J

Databases (2001-2008) for cases in which recombinant bone morphogenetic protein (rhBMP) was used to aid in management of orthopedic disease were reviewed and cases were categorized as non-unions, delayed unions, and cases expected to heal with difficulty. If follow-up in the medical record was < 6 mo for live animals, owners were surveyed by telephone. Thirteen cases (11 dogs, 2 cats) were identified; OP-1 (rhBMP-7) was used in 3 cases and INFUSE (rhBMP-2) in 10.

Authors: Herten M, Grassmann JP, Sager M, Benga L, Fischer JC, Jäger M, Betsch M, Wild M, Hakimi M, Jungbluth P.
Journal: VCOT

Autologous bone marrow plays an increasing role in the treatment of bone, cartilage and tendon healing disorders. Cell-based therapies display promising results in the support of local regeneration, especially therapies using intra-operative one-step treatments with autologous progenitor cells. In the present study, bone marrow-derived cells were concentrated in a point-of-care device and investigated for their mesenchymal stem cell (MSC) characteristics and their osteogenic potential.Bone marrow was harvested from the iliac crest of 16 minipigs.

Category: Biologics