Antibiotic delivery

OBJECTIVE: To compare the gross and histological effects of intravenous regional limb perfusion (IVRLP) using amikacin sulfate on second intention healing of distal limb wounds in adult horses with healing in untreated wounds.

Authors: Alanna J. Zantingh, Wayne S. Schwark, Susan L. Fubini and Ashlee E. Watts
Journal: Veterinary Surgery

Objectives

To determine the effect of regional limb perfusion (RLP) with amikacin sulfate alone and in combination with ticarcillin/clavulanate on synovial fluid concentration and antimicrobial activity of amikacin.
Sample Population

Experimental study.
Methods

Authors: A. T. Mahne, E. Rioja, H. J. Marais, N. F. Villarino and L. M. Rubio-Martinez
Journal: Equine Veterinary Journal

Reasons for performing study

Antimicrobial i.v. regional limb perfusion (IV-RLP) is clinically performed on anaesthetised or sedated horses with or without regional anaesthesia. To date, no scientific data are available on the clinical and pharmacokinetic effects of these anaesthetic protocols on antimicrobial IV-RLP, which is believed to result in better tourniquet efficiency due to decreased movement.
Objective

To determine the effects of regional or general anaesthesia on the clinical and synovial pharmacokinetic parameters of amikacin administered by IV-RLP to horses.

Authors: P. Trencart, Y. A. Elce, E. Rodriguez Batista, G. Michaud
Journal: Veterinary and Comparative Orthopaedics and Traumatology

Introduction: Ethylene oxide is currently recommended for sterilization of antibiotic impregnated beads; however this method carries health risks to personnel and is becoming less available. Objective: To perform a pilot study of the effect of radiation for sterilization of polymethylmethacrylate (PMMA) and plaster of Paris (POP) beads impregnated with amikacin, enrofloxacin, and ceftiofur. Hypothesis: Radiation would effectively sterilize the beads without affecting the efficacy of the antibiotic.

Authors: Elodie Lallemand, Pierre Trencart, Carine Tahier, Frederic Dron, Angelique Paulin and Caroline Tessier
Journal: Veterinary Surgery

Objective

To evaluate pharmacokinetic–pharmacodynamic variables and local tolerance at injection-site of marbofloxacin administered via regional intravenous limb perfusion (RIVLP) in standing horses.
Animals

Adult horses (n = 6).
Methods

Authors: Luis M. Rubio-Martínez, Colette R. Elmas, Belinda Black, Gabrielle Monteith
Journal: Journal of the American Veterinary Medical Association

Objective—To describe the clinical use of regional limb perfusion with antimicrobials (A-RLP), complications, and outcome in a large series of patients.

Design—Retrospective case series.

Animals—174 horses.

Authors: Gal Kelmer, Amos Tatz and Tali Bdolah-Abram
Journal: Veterinary Surgery

Objective

To describe indwelling cephalic or saphenous vein catheter use for intravenous regional limb perfusion (ID-IV-RLP) to treat horses with synovial injury (contamination/infection) of the distal aspect of the limb.
Study Design

Retrospective case series.
Animals

Horses (n = 44; 45 limbs) treated with proximal ID-IV-RLP.
Methods

Authors: A. F. Sanchez Teran, L. M. Rubio-Martinez, N. F. Villarino and M. G. Sanz
Journal: Equine Veterinary Journal

Summary

Reasons for performing study

Serum amyloid A (SAA) in synovial fluid has recently been used as a marker for septic arthritis in horses but the effects of repeated intra-articular (IA) administration of amikacin on synovial SAA concentrations are unknown.

Objectives

To report the effect of repeated IA administration of amikacin on SAA, total protein (TP), nucleated cell count (NCC) and differential NCC in synovial fluid of healthy equine joints.

Methods

Authors: Axel M. Beccar-Varela MV, Kira L. Epstein DVM, Diplomate ACVS, Catherine L. White PhD
Journal: Veterinary Surgery

Objectives
To determine the effects of experimentally induced synovitis of the radiocarpal joint on the intra-articular pharmacokinetics and pharmacodynamics of amikacin after intravenous regional limb perfusion (IVRLP).
Study Design
Randomized crossover experimental design.
Animals
Adult horses (n = 8).
Methods

Authors: Leslie A. Thomas DVM, Tatiana Bizikova MSc, Anne C. Minihan DVM, Diplomate ACVS
Journal: Veterinary Surgery

Objective: To characterize the in vitro elution and bioactivity of 2 formulations of antibiotics in a novel, dissolvable, cross-linked dextran polymer matrix: Formulation 1—amikacin and clindamycin (AC); Formulation 2—amikacin, clindamycin, and vancomycin (ACV).

Study Design: Prospective, in vitro, experimental study.